Composite

Part:BBa_K5061034:Design

Designed by: Florent Cha   Group: iGEM24_Evry-Paris-Saclay   (2024-09-14)


Test Plasmid (TP) Golden Gate cloning platform


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3042
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3042
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3042
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3042
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 18
    Illegal BsaI.rc site found at 6


Design Notes

Source

DNA synthesis, PCR amplifications and Golden Gate assembly

References